Princeton University

School of Engineering & Applied Science

Towards Personal Health Monitoring: Detecting Biomolecules with Microfluids and Nanoplasmonic Sensors Fabricated by Nanoimprint Lithography

Ruoming Peng
Engineering Quadrangle J401
Thursday, June 8, 2017 - 10:00am to 11:30am

The research works will be presented in this talk include the design, fabrication, and characterization of nanostructures for biosensing applications. The detection of proteins and nucleic acids with nanoplasmonic sensors in multiple formats (conventional 96-well plate, micro/nanofluidics) will be discussed. Several related topics will also be addressed: superposition of elementary nanostructures to form complex structures with an advanced multi-nanoimprint lithography (MNIL) fabrication method; and the integration of nanoplasmonic sensors with a microfluidic chip to make the chip a point-of-care (POC) device.
The first part of this talk will cover the applications of disk-coupled dots-on-pillar (D2PA) in deoxyribonucleic acid (DNA) detection using the fluorescent DNA hybridization assay. The systematic protocol optimization and novel biomolecular quantification method of a fluorescent DNA hybridization assay will be discussed in detail. With these, a record-high limit of detection (LoD) is achieved for the fluorescent DNA hybridization assay.
The second part of the talk will shift gear to discuss the integration of D2PA into microfluidic chips to fulfill Point-of-Care (POC) applications. The introduction of microfluidic channels into this assay system can drastically reduce the required assay time and quantities of reagents. The sample delivery is driven by the capillary force and requires no external power, which makes the POC device easy to use.
The last part of this talk will introduce a multi-nanoimprint lithography (MNIL) method to fabricate nanofluidic sensor with a single line of D2PA inside each nanochannel. The sensor can potentially be used in DNA stretch and detection due to the nanoscale confinement.